A rapid, sensitive and specific RP-HPLC method involving U.V. detection was developed and validated for the estimation of Cefixime and Linezolid in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity, robustness, limit of detection and limit of quantitation. The mobile phase used methanol: phosphate buffer in the ratio of 60:40 and pH adjusted to 3.5 with orthophosphoric acid. The detection of combined dosage form was carried out at 278 nm at constant flow rate of 1 ml/min. The retention time of Cefixime and Linezolid were found 3.304 min and 6.447 min respectively. Linearity was observed in 4-16 μg/ml for Cefixime (r2=0.998) and 12-48 μg/ml for Linezolid (r2=0.999). Detection limit for Cefixime and Linezolid is 0.159 μg/ml and 0.847 μg/ml respectively and quantification limit for Cefixime and Linezolid is 0.483 μg/ml and 2.566 μg/ml. The proposed method was successfully applied for the quantitative determination of Cefixime and Linezolid in tablet dosage form.
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